I performed Dna Extraction experiment.
Procedure:
- Took 10ml water and gargled to extract cheek cells.
- Added 4 drops of dishwash liquid and 2 spoons of salt.
- Mixed well to form homogenous solution
- Waited for 2 minutes for soap and salt to tear apart lipid membranes in cells exposing DNA of cheek cells and obviously some bacteria present in mouth.
- Now I added equal amount of chill sanitizer liquid.
Observations:
- Formation of white precipitate(DNA is insoluble in alcohol).
- That precipitate formed thread like structure.
- This is DNA precipitate.
Notes:
- Adding equal amount of chilled sanitizer(alcohol) is important for precipitation.
- First I tried to extract DNA from tomato pulp. I could see cloudy precipitate but the amount was not enough to form strings. so taking a pulpy fruit in sufficient quantity would be better instead of berries which tend to be more of water rather than cells.
- Do not dilute the solution too much with water.
Cube Homelab Warangal, DNA Extraction at home with Deekshith.
I invite collaborators to try out this experiment and share their insights and experiences.
Thankyou for reading!
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